Intein splicing is the process by which inteins, or intervening proteins, are removed from precursor proteins, allowing the remaining protein fragments, known as exteins, to be ligated together. Inteins are found within protein-coding sequences and are able to catalyze their own removal from the precursor protein through a series of chemical reactions. This process is important in the post-translational modification of proteins and has applications in biotechnology and protein engineering. Intein splicing has been extensively studied to understand its mechanisms and potential uses in manipulating protein sequences.